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  2. FCEN
  3. FCEN - Facultad de Ciencias Exactas y Naturales. UBA
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dc.provenanceFacultad de Ciencias Exactas y Naturales de la UBA-
dc.contributor<div class="autor_fcen" id="5604">Maymó, J.L.</div>-
dc.contributorPérez Pérez, A.-
dc.contributorMaskin, B.-
dc.contributorDueñas, J.L.-
dc.contributor<div class="autor_fcen" id="1356">Calvo, J.C.</div>-
dc.contributorSánchez Margalet, V.-
dc.contributor<div class="autor_fcen" id="8831">Varone, C.L.</div>-
dc.creator<div class="autor_fcen" id="5604">Maymó, J.L.</div>-
dc.creatorPérez Pérez, A.-
dc.creatorMaskin, B.-
dc.creatorDueñas, J.L.-
dc.creator<div class="autor_fcen" id="1356">Calvo, J.C.</div>-
dc.creatorSánchez Margalet, V.-
dc.creator<div class="autor_fcen" id="8831">Varone, C.L.</div>-
dc.date.accessioned2018-05-04T22:00:45Z-
dc.date.accessioned2018-05-28T15:49:09Z-
dc.date.available2018-05-04T22:00:45Z-
dc.date.available2018-05-28T15:49:09Z-
dc.date.issued2012-
dc.identifier.urihttp://10.0.0.11:8080/jspui/handle/bnmm/68612-
dc.descriptionPleiotropic effects of leptin have been identified in reproduction and pregnancy, particularly in the placenta, where it works as an autocrine hormone. In this work, we demonstrated that human chorionic gonadotropin (hCG) added to JEG-3 cell line or to placental explants induces endogenous leptin expression. We also found that hCG increased cAMP intracellular levels in BeWo cells in a dose-dependent manner, stimulated cAMP response element (CRE) activity and the cotransfection with an expression plasmid of a dominant negative mutant of CREB caused a significant inhibition of hCG stimulation of leptin promoter activity. These results demonstrate that hCG indeed activates cAMP/PKA pathway, and that this pathway is involved in leptin expression. Nevertheless, we found leptin induction by hCG is dependent on cAMP levels. Treatment with (Bu)2cAMP in combination with low and non stimulatory hCG concentrations led to an increase in leptin expression, whereas stimulatory concentrations showed the opposite effect. We found that specific PKA inhibition by H89 caused a significant increase of hCG leptin induction, suggesting that probably high cAMP levels might inhibit hCG effect. It was found that hCG enhancement of leptin mRNA expression involved the MAPK pathway. In this work, we demonstrated that hCG leptin induction through the MAPK signaling pathway is inhibited by PKA. We observed that ERK1/2 phosphorylation increased when hCG treatment was combined with H89. In view of these results, the involvement of the alternative cAMP/Epac signaling pathway was studied. We observed that a cAMP analogue that specifically activates Epac (CPT-OMe) stimulated leptin expression by hCG. In addition, the overexpression of Epac and Rap1 proteins increased leptin promoter activity and enhanced hCG. In conclusion, we provide evidence suggesting that hCG induction of leptin gene expression in placenta is mediated not only by activation of the MAPK signaling pathway but also by the alternative cAMP/Epac signaling pathway. © 2012 Maymó et al.-
dc.descriptionFil:Maymó, J.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.descriptionFil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.descriptionFil:Varone, C.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.formatapplication/pdf-
dc.languageeng-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttp://creativecommons.org/licenses/by/2.5/ar-
dc.sourcePLoS ONE 2012;7(10)-
dc.source.urihttp://digital.bl.fcen.uba.ar/Download/paper/paper_19326203_v7_n10_p_Maymo.pdf-
dc.subjectchorionic gonadotropin-
dc.subjectcyclic AMP-
dc.subjectcyclic AMP dependent protein kinase-
dc.subjectcyclic AMP dependent protein kinase inhibitor-
dc.subjectcyclic AMP derivative-
dc.subjectcyclic AMP responsive element binding protein-
dc.subjectEPAC protein-
dc.subjectexchange protein directly activated by cyclic AMP-
dc.subjectleptin-
dc.subjectmessenger RNA-
dc.subjectmitogen activated protein kinase-
dc.subjectmitogen activated protein kinase 1-
dc.subjectn [2 (4 bromocinnamylamino)ethyl] 5 isoquinolinesulfonamide-
dc.subjectprotein-
dc.subjectRap1 protein-
dc.subjectunclassified drug-
dc.subjectarticle-
dc.subjectBewo cell-
dc.subjectcell line-
dc.subjectcell strain JEG 3-
dc.subjectcontrolled study-
dc.subjectcyclic AMP responsive element-
dc.subjectenzyme activation-
dc.subjectenzyme inhibition-
dc.subjectenzyme phosphorylation-
dc.subjectexplant-
dc.subjectgene activity-
dc.subjectgenetic transfection-
dc.subjecthuman-
dc.subjecthuman cell-
dc.subjecthuman tissue-
dc.subjectplacenta-
dc.subjectplasmid-
dc.subjectpromoter region-
dc.subjectprotein expression-
dc.subjectprotein induction-
dc.subjectsignal transduction-
dc.subjectBase Sequence-
dc.subjectBlotting, Western-
dc.subjectCell Line, Tumor-
dc.subjectChorionic Gonadotropin-
dc.subjectCyclic AMP-
dc.subjectCyclic AMP-Dependent Protein Kinases-
dc.subjectDNA Primers-
dc.subjectEnzyme Activation-
dc.subjectFemale-
dc.subjectGuanine Nucleotide Exchange Factors-
dc.subjectHumans-
dc.subjectLeptin-
dc.subjectMAP Kinase Signaling System-
dc.subjectPlacenta-
dc.subjectPregnancy-
dc.subjectReal-Time Polymerase Chain Reaction-
dc.titleThe Alternative Epac/cAMP Pathway and the MAPK Pathway Mediate hCG Induction of Leptin in Placental Cells-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:ar-repo/semantics/artículo-
dc.typeinfo:eu-repo/semantics/publishedVersion-
Aparece en las colecciones: FCEN - Facultad de Ciencias Exactas y Naturales. UBA

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