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dc.provenanceFacultad de Ciencias Exactas y Naturales de la UBA-
dc.contributorNikel, P.I.-
dc.contributorGiordano, A.M.-
dc.contributor<div class="autor_fcen" id="2305">De Almeida, A.</div>-
dc.contributorGodoy, M.S.-
dc.contributor<div class="autor_fcen" id="6704">Pettinari, M.J.</div>-
dc.creatorNikel, P.I.-
dc.creatorGiordano, A.M.-
dc.creator<div class="autor_fcen" id="2305">De Almeida, A.</div>-
dc.creatorGodoy, M.S.-
dc.creator<div class="autor_fcen" id="6704">Pettinari, M.J.</div>-
dc.date.accessioned2018-05-04T22:04:46Z-
dc.date.accessioned2018-05-28T15:48:16Z-
dc.date.available2018-05-04T22:04:46Z-
dc.date.available2018-05-28T15:48:16Z-
dc.date.issued2010-
dc.identifier.urihttp://10.0.0.11:8080/jspui/handle/bnmm/68454-
dc.descriptionThe effect of eliminating D-lactate synthesis in poly(3-hydroxybutyrate) (PHB)-accumulating recombinant Escherichia coli (K24K) was analyzed using glycerol as a substrate. K24KL, an ldhA derivative, produced more biomass and had altered carbon partitioning among the metabolic products, probably due to the increased availability of carbon precursors and reducing power. This resulted in a significant increase of PHB and ethanol synthesis and a decrease in acetate production. Cofactor measurements revealed that cultures of K24K and K24KL had a high intracellular NADPH content and that the NADPH/NADP+ ratio was higher than the NADH/NAD+ ratio. The ldhA mutation affected cofactor distribution, resulting in a more reduced intracellular state, mainly due to a further increase in NADPH/NADP+. In 60-h fed-batch cultures, K24KL reached 41.9 g · liter-1 biomass and accumulated PHB up to 63% ± 1% (wt/wt), with a PHB yield on glycerol of 0.41 ± 0.03 g · g-1, the highest reported using this substrate. © 2010, American Society for Microbiology.-
dc.descriptionFil:De Almeida, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.descriptionFil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.formatapplication/pdf-
dc.languageeng-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttp://creativecommons.org/licenses/by/2.5/ar-
dc.sourceAppl. Environ. Microbiol. 2010;76(22):7400-7406-
dc.source.urihttp://digital.bl.fcen.uba.ar/Download/paper/paper_00992240_v76_n22_p7400_Nikel.pdf-
dc.subjectCarbon partitioning-
dc.subjectCarbon precursors-
dc.subjectCofactors-
dc.subjectD-lactate-
dc.subjectFed-batch cultures-
dc.subjectMetabolic products-
dc.subjectPoly(3-hydroxybutyrate)-
dc.subjectRecombinant Escherichia coli-
dc.subjectReducing power-
dc.subjectBatch cell culture-
dc.subjectEscherichia coli-
dc.subjectEthanol-
dc.subjectSubstrates-
dc.subjectGlycerol-
dc.subjectalcohol-
dc.subjectcarbon-
dc.subjectglycerol-
dc.subjecthydroxybutyric acid-
dc.subjectlactate dehydrogenase-
dc.subjectlactic acid-
dc.subjectnicotinamide adenine dinucleotide-
dc.subjectnicotinamide adenine dinucleotide phosphate-
dc.subjectpoly(3 hydroxybutyric acid)-
dc.subjectpoly-beta-hydroxybutyrate-
dc.subjectpolyester-
dc.subjectalcohol-
dc.subjectbiomass-
dc.subjectester-
dc.subjectethanol-
dc.subjectfecal coliform-
dc.subjectpartitioning-
dc.subjectrecombination-
dc.subjectarticle-
dc.subjectbiomass-
dc.subjectbioreactor-
dc.subjectchemistry-
dc.subjectEscherichia coli-
dc.subjectgenetics-
dc.subjectgrowth, development and aging-
dc.subjectmetabolism-
dc.subjecttime-
dc.subjectBiomass-
dc.subjectBioreactors-
dc.subjectCarbon-
dc.subjectEscherichia coli-
dc.subjectEthanol-
dc.subjectGlycerol-
dc.subjectHydroxybutyrates-
dc.subjectLactate Dehydrogenases-
dc.subjectLactic Acid-
dc.subjectMetabolic Networks and Pathways-
dc.subjectNAD-
dc.subjectNADP-
dc.subjectPolyesters-
dc.subjectTime Factors-
dc.subjectEscherichia coli-
dc.titleElimination of D-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant Escherichia coli-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:ar-repo/semantics/artículo-
dc.typeinfo:eu-repo/semantics/publishedVersion-
Aparece en las colecciones: FCEN - Facultad de Ciencias Exactas y Naturales. UBA

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