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dc.provenanceFacultad de Ciencias Exactas y Naturales de la UBA-
dc.contributorNikel, P.I.-
dc.contributor<div class="autor_fcen" id="6704">Pettinari, M.J.</div>-
dc.contributor<div class="autor_fcen" id="3442">Galvagno, M.A.</div>-
dc.contributor<div class="autor_fcen" id="5666">Méndez, B.S.</div>-
dc.creatorNikel, P.I.-
dc.creator<div class="autor_fcen" id="6704">Pettinari, M.J.</div>-
dc.creator<div class="autor_fcen" id="3442">Galvagno, M.A.</div>-
dc.creator<div class="autor_fcen" id="5666">Méndez, B.S.</div>-
dc.date.accessioned2018-05-04T22:03:44Z-
dc.date.accessioned2018-05-28T15:48:14Z-
dc.date.available2018-05-04T22:03:44Z-
dc.date.available2018-05-28T15:48:14Z-
dc.date.issued2006-
dc.identifier.urihttp://10.0.0.11:8080/jspui/handle/bnmm/68450-
dc.descriptionWe assessed the effects of different arcA mutations on poly(3- hydroxybutyrate) (PHB) synthesis in recombinant Escherichia coli strains carrying the pha synthesis genes from Azotobacter sp. strain FA8. The arcA mutations used were an internal deletion and the arcA2 allele, a leaky mutation for some of the characteristics of the Arc phenotype which confers high respiratory capacity. PHB synthesis was not detected in the wild-type strain in shaken flask cultures under low-oxygen conditions, while ArcA mutants gave rise to polymer accumulation of up to 24% of their cell dry weight. When grown under microaerobic conditions in a bioreactor, the arcA deletion mutant reached a PHB content of 27% ± 2%. Under the same conditions, higher biomass and PHB concentrations were observed for the strain bearing the arcA2 allele, resulting in a PHB content of 35% ± 3%. This strain grew in a simple medium at a specific growth rate of 0.69 ± 0.07 h-1, whereas the deletion mutant needed several nutritional additives and snowed a specific growth rate of 0.56 ± 0.06 h-1. The results presented here suggest that arcA mutations could play a role in heterologous PHB synthesis in microaerobiosis. Copyright © 2006, American Society for Microbiology. All Rights Reserved.-
dc.descriptionFil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.descriptionFil:Galvagno, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.descriptionFil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.formatapplication/pdf-
dc.languageeng-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttp://creativecommons.org/licenses/by/2.5/ar-
dc.sourceAppl. Environ. Microbiol. 2006;72(4):2614-2620-
dc.source.urihttp://digital.bl.fcen.uba.ar/Download/paper/paper_00992240_v72_n4_p2614_Nikel.pdf-
dc.subjectBiomass-
dc.subjectCell culture-
dc.subjectEscherichia coli-
dc.subjectMutagenesis-
dc.subjectStrain-
dc.subjectSynthesis (chemical)-
dc.subjectArcA mutants-
dc.subjectLeaky mutation-
dc.subjectMicroaerobiosis-
dc.subjectPoly(3-hydroxybutyrate) synthesis-
dc.subjectOrganic polymers-
dc.subjectpoly(3 hydroxybutyric acid)-
dc.subjectbacteriology-
dc.subjectgene-
dc.subjectmutagenicity-
dc.subjectrecombination-
dc.subjectaerobic metabolism-
dc.subjectallele-
dc.subjectarcA gene-
dc.subjectarcA2 gene-
dc.subjectarticle-
dc.subjectAzotobacter-
dc.subjectAzotobacter FA8-
dc.subjectbacterial gene-
dc.subjectbacterial growth-
dc.subjectbacterial strain-
dc.subjectbiomass-
dc.subjectbioreactor-
dc.subjectdry weight-
dc.subjectEscherichia coli-
dc.subjectgene deletion-
dc.subjectgene mutation-
dc.subjectnonhuman-
dc.subjectpha gene-
dc.subjectphenotype-
dc.subjectsynthesis-
dc.subjectwild type-
dc.subjectAerobiosis-
dc.subjectBacterial Outer Membrane Proteins-
dc.subjectBiomass-
dc.subjectCulture Media-
dc.subjectEscherichia coli-
dc.subjectEscherichia coli Proteins-
dc.subjectGene Expression Regulation, Bacterial-
dc.subjectHydroxybutyrates-
dc.subjectMutation-
dc.subjectPolyesters-
dc.subjectRecombination, Genetic-
dc.subjectRepressor Proteins-
dc.subjectArca-
dc.subjectAzotobacter-
dc.subjectBacteria (microorganisms)-
dc.subjectEscherichia coli-
dc.titlePoly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:ar-repo/semantics/artículo-
dc.typeinfo:eu-repo/semantics/publishedVersion-
Aparece en las colecciones: FCEN - Facultad de Ciencias Exactas y Naturales. UBA

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